This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kerby, R. L.
Right arrow Articles by Roberts, G. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kerby, R. L.
Right arrow Articles by Roberts, G. P.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2008, p. 3336-3343, Vol. 190, No. 9
0021-9193/08/$08.00+0     doi:10.1128/JB.00033-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

RcoM: A New Single-Component Transcriptional Regulator of CO Metabolism in Bacteria{triangledown}

Robert L. Kerby, Hwan Youn, and Gary P. Roberts*

Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 8 January 2008/ Accepted 19 February 2008

Genomic analysis suggested the existence of a CO-sensing bacterial transcriptional regulator that couples an N-terminal PAS fold domain to a C-terminal DNA-binding LytTR domain. UV/visible-light spectral analyses of heterologously expressed, purified full-length proteins indicated that they contained a hexacoordinated b-type heme moiety that avidly binds CO and NO. Studies of protein variants strongly suggested that the PAS domain residues His74 and Met104 serve as the heme Fe(II) axial ligands, with displacement of Met104 upon binding of the gaseous effectors. Two RcoM (regulator of CO metabolism) homologs were shown to function in vivo as CO sensors capable of regulating an aerobic CO oxidation (cox) regulon. The genetic linkage of rcoM with both aerobic (cox) and anaerobic (coo) CO oxidation systems suggests that in different organisms RcoM proteins may control either regulon type.

* Corresponding author. Mailing address: 1550 Linden Dr., Madison, WI 53706. Phone: (608) 262-3567. Fax: (608) 262-9865. E-mail: groberts{at}bact.wisc.edu

{triangledown} Published ahead of print on 7 March 2008.

Journal of Bacteriology, May 2008, p. 3336-3343, Vol. 190, No. 9
0021-9193/08/$08.00+0     doi:10.1128/JB.00033-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»