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Journal of Bacteriology, January 2009, p. 109-114, Vol. 191, No. 1
0021-9193/09/$08.00+0     doi:10.1128/JB.01166-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Geobacter sulfurreducens Contains Separate C- and A-Adding tRNA Nucleotidyltransferases and a Poly(A) Polymerase{triangledown} ,{dagger}

Patricia Bralley, Madeline Cozad, and George H. Jones*

Department of Biology, Emory University, Atlanta, Georgia 30319

Received 18 August 2008/ Accepted 16 October 2008

The genome of Geobacter sulfurreducens contains three genes whose sequences are quite similar to sequences encoding known members of an RNA nucleotidyltransferase superfamily that includes tRNA nucleotidyltransferases and poly(A) polymerases. Reverse transcription-PCR using G. sulfurreducens total RNA demonstrated that the genes encoding these three proteins are transcribed. These genes, encoding proteins designated NTSFI, NTSFII, and NTSFIII, were cloned and overexpressed in Escherichia coli. The corresponding enzymes were purified and assayed biochemically, resulting in identification of NTSFI as a poly(A) polymerase, NTSFII as a C-adding tRNA nucleotidyltransferase, and NTSFIII as an A-adding tRNA nucleotidyltransferase. Analysis of G. sulfurreducens rRNAs and mRNAs revealed the presence of heteropolymeric RNA 3' tails. This is the first characterization of a bacterial system that expresses separate C- and A-adding tRNA nucleotidyltransferases and a poly(A) polymerase.

* Corresponding author. Mailing address: Department of Biology, Emory University, Atlanta, GA 30319. Phone: (404) 727-0712. Fax: (404) 727-2880. E-mail: george.h.jones{at}emory.edu

{triangledown} Published ahead of print on 24 October 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, January 2009, p. 109-114, Vol. 191, No. 1
0021-9193/09/$08.00+0     doi:10.1128/JB.01166-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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