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Journal of Bacteriology, January 2009, p. 310-319, Vol. 191, No. 1
0021-9193/09/$08.00+0     doi:10.1128/JB.00513-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

SMC Protein-Dependent Chromosome Condensation during Aerial Hyphal Development in Streptomyces{triangledown} ,{dagger}

Agnieszka Kois,1,2 Magdalena Swiatek,2 Dagmara Jakimowicz,1,2 and Jolanta Zakrzewska-Czerwinska1,2*

Faculty of Biotechnology, University of Wroclaw, ul. Tamka 2, 50-137 Wroclaw, Poland,1 Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114 Wroclaw, Poland2

Received 15 April 2008/ Accepted 3 October 2008

Members of the SMC (structural maintenance of chromosomes) protein family play a central role in higher-order chromosome dynamics from bacteria to humans. So far, studies of bacterial SMC proteins have focused only on unicellular rod-shaped organisms that divide by binary fission. The conversion of multigenomic aerial hyphae of the mycelial organism Streptomyces coelicolor into chains of unigenomic spores requires the synchronous segregation of multiple chromosomes. Here we focus on the contribution of SMC proteins to sporulation-associated chromosome segregation in S. coelicolor. Deletion of the smc gene causes aberrant DNA condensation and missegregation of chromosomes (7.5% anucleate spores). In vegetative mycelium, immunostained SMC proteins were observed sporadically, while in aerial hyphae about to undergo sporulation they appeared as irregularly spaced foci which accompanied but did not colocalize with ParB complexes. Our data demonstrate that efficient chromosome segregation requires the joint action of SMC and ParB proteins. SMC proteins, similarly to ParAB and FtsZ, presumably belong to a larger group of proteins whose expression is highly induced in response to the requirement of aerial hyphal maturation.

* Corresponding author. Mailing address: Faculty of Biotechnology, University of Wroclaw, Tamka 2, 50-137 Wroclaw, Poland. Phone: 48 71 3752502. Fax: 48 71 3752608. E-mail: zakrzewska{at}protein.pl

{triangledown} Published ahead of print on 17 October 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, January 2009, p. 310-319, Vol. 191, No. 1
0021-9193/09/$08.00+0     doi:10.1128/JB.00513-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Salerno, P., Larsson, J., Bucca, G., Laing, E., Smith, C. P., Flardh, K. (2009). One of the Two Genes Encoding Nucleoid-Associated HU Proteins in Streptomyces coelicolor Is Developmentally Regulated and Specifically Involved in Spore Maturation. J. Bacteriol. 191: 6489-6500 [Abstract] [Full Text]  
  • Dedrick, R. M., Wildschutte, H., McCormick, J. R. (2009). Genetic Interactions of smc, ftsK, and parB Genes in Streptomyces coelicolor and Their Developmental Genome Segregation Phenotypes. J. Bacteriol. 191: 320-332 [Abstract] [Full Text]  


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