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Journal of Bacteriology, January 2009, p. 422-427, Vol. 191, No. 1
0021-9193/09/$08.00+0     doi:10.1128/JB.01263-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genomic Characterization of Ralstonia solanacearum Phage {phi}RSB1, a T7-Like Wide-Host-Range Phage{triangledown} ,{dagger}

Takeru Kawasaki, Mio Shimizu, Hideki Satsuma, Akiko Fujiwara, Makoto Fujie, Shoji Usami, and Takashi Yamada*

Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan

Received 9 September 2008/ Accepted 15 October 2008

{phi}RSB1 is a wide-host-range, T7-like bacteriophage that infects and efficiently lyses the phytopathogenic bacterium Ralstonia solanacearum. The {phi}RSB1 genome comprises 43,079 bp of double-stranded DNA (61.7% G+C) with 325-bp terminal repeats and contains 47 open reading frames. Strong activity of tandem early promoters and wide specificity of phage promoters of {phi}RSB1 were demonstrated.

* Corresponding author. Mailing address: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan. Phone and fax: 81-82-424-7752. E-mail: tayamad{at}hiroshima-u.ac.jp

{triangledown} Published ahead of print on 24 October 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, January 2009, p. 422-427, Vol. 191, No. 1
0021-9193/09/$08.00+0     doi:10.1128/JB.01263-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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