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Journal of Bacteriology, May 2009, p. 3403-3406, Vol. 191, No. 10
0021-9193/09/$08.00+0     doi:10.1128/JB.00022-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

A Novel Nitroreductase of Staphylococcus aureus with S-Nitrosoglutathione Reductase Activity{triangledown} ,{dagger}

Ana Filipa N. Tavares,1,{ddagger} Lígia S. Nobre,1,{ddagger} Ana M. P. Melo,2,3 and Lígia M. Saraiva1*

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República (EAN), 2780-157 Oeiras, Portugal,1 ECO-BIO, Instituto de Investigação Científica Tropical, Av. da República (EAN), 2784-505 Oeiras, Portugal,2 Faculdade de Engenharia e Ciências Naturais, Universidade Lusófona de Humanidades e Tecnologias, Av. do Campo Grande, 376, 1749-024 Lisbon, Portugal3

Received 8 January 2009/ Accepted 27 February 2009

In this report we show that inactivation of the putative nitroreductase SA0UHSC_00833 (ntrA) increases the sensitivity of Staphylococcus aureus to S-nitrosoglutathione (GSNO) and augments its resistance to nitrofurans. S. aureus NtrA is a bifunctional enzyme that exhibits nitroreductase and GSNO reductase activity. A phylogenetic analysis suggests that NtrA is a member of a novel family of nitroreductases that seems to play a dual role in vivo, promoting nitrofuran activation and protecting the cell against transnitrosylation.

* Corresponding author. Mailing address: Instituto de Tecnologia Química e Biológica, UNL, Av. da República (EAN), 2780-157 Oeiras, Portugal. Phone: 351-214469328. Fax: 351-214411277. E-mail: lst{at}itqb.unl.pt

{triangledown} Published ahead of print on 13 March 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} A.F.N.T. and L.S.N. contributed equally to the work.

Journal of Bacteriology, May 2009, p. 3403-3406, Vol. 191, No. 10
0021-9193/09/$08.00+0     doi:10.1128/JB.00022-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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