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Journal of Bacteriology, June 2009, p. 3677-3684, Vol. 191, No. 11
0021-9193/09/$08.00+0     doi:10.1128/JB.01763-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

In Vivo Interactions between Toxin-Antitoxin Proteins Epsilon and Zeta of Streptococcal Plasmid pSM19035 in Saccharomyces cerevisiae{triangledown} ,{dagger}

Urszula Zielenkiewicz,1* Magdalena Kowalewska,2 Celina Kaczor,1,3 and Piotr Ceglowski1

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland,1 Department of Molecular Biology, The Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology, W.K. Roentgena 5, 02-781 Warsaw, Poland,2 Warsaw University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, Poland3

Received 16 December 2008/ Accepted 26 March 2009

The widespread prokaryotic toxin-antitoxin (TA) systems involve conditional interaction between two TA proteins. The interaction between the Epsilon and Zeta proteins, constituting the TA system of plasmid pSM19035 from Streptococcus pyogenes, was detected in vivo using a yeast two-hybrid system. As we showed using Saccharomyces cerevisiae, the Zeta toxin hybrid gene also exerts its toxic effects in a dose-dependent manner in eukaryotic cells. Analysis of mutant proteins in the two-hybrid system demonstrated that the N-terminal part of Zeta and the N-terminal region of Epsilon are involved in the interaction. The N-terminal region of the Zeta protein and its ATP/GTP binding motif were found to be responsible for the toxicity.

* Corresponding author. Mailing address: Institute of Biochemistry and Biophysics, PAS, Pawinskiego 5A, 02-106 Warsaw, Poland. Phone: (48) 225921206. Fax: (48) 226584636. E-mail: ulazet{at}ibb.waw.pl

{triangledown} Published ahead of print on 3 April 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, June 2009, p. 3677-3684, Vol. 191, No. 11
0021-9193/09/$08.00+0     doi:10.1128/JB.01763-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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