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Journal of Bacteriology, July 2009, p. 4082-4096, Vol. 191, No. 13
0021-9193/09/$08.00+0     doi:10.1128/JB.01540-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of OscR, a Transcriptional Regulator Involved in Osmolarity Adaptation in Vibrio cholerae{triangledown} ,{dagger}

Nicholas J. Shikuma and Fitnat H. Yildiz*

Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California 95064

Received 30 October 2008/ Accepted 21 March 2009

Vibrio cholerae is a facultative human pathogen. In its aquatic habitat and as it passes through the digestive tract, V. cholerae must cope with fluctuations in salinity. We analyzed the genome-wide transcriptional profile of V. cholerae grown at different NaCl concentrations and determined that the expression of compatible solute biosynthesis and transporter genes, virulence genes, and genes involved in adhesion and biofilm formation is differentially regulated. We determined that salinity modulates biofilm formation, and this response was mediated through the transcriptional regulators VpsR and VpsT. Additionally, a transcriptional regulator controlling an osmolarity adaptation response was identified. This regulator, OscR (osmolarity controlled regulator), was found to modulate the transcription of genes involved in biofilm matrix production and motility in a salinity-dependent manner. oscR mutants were less motile and exhibited enhanced biofilm formation only under low-salt conditions.

* Corresponding author. Mailing address: Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, CA 95064. Phone: (831) 459-1588. Fax: (831) 459-3524. E-mail: yildiz{at}metx.ucsc.edu

{triangledown} Published ahead of print on 27 March 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, July 2009, p. 4082-4096, Vol. 191, No. 13
0021-9193/09/$08.00+0     doi:10.1128/JB.01540-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Related articles in JB:

OscR, a New Osmolarity-Responsive Regulator in Vibrio cholerae
Paul V. Dunlap
JB 2009 191: 4053-4055. [Full Text]  



This article has been cited by other articles:

  • Shikuma, N. J., Fong, J. C. N., Odell, L. S., Perchuk, B. S., Laub, M. T., Yildiz, F. H. (2009). Overexpression of VpsS, a Hybrid Sensor Kinase, Enhances Biofilm Formation in Vibrio cholerae. J. Bacteriol. 191: 5147-5158 [Abstract] [Full Text]  
  • Dunlap, P. V. (2009). OscR, a New Osmolarity-Responsive Regulator in Vibrio cholerae. J. Bacteriol. 191: 4053-4055 [Full Text]  


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