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Journal of Bacteriology, July 2009, p. 4687-4692, Vol. 191, No. 14
0021-9193/09/$08.00+0     doi:10.1128/JB.00421-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of a Catabolite-Responsive Element Necessary for Regulation of the cry4A Gene of Bacillus thuringiensis subsp. israelensis{triangledown} ,{dagger}

Sashi Kant,1 Rupam Kapoor,2 and Nirupama Banerjee1*

International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067,1 Department of Botany, University of Delhi, New Delhi 1100072

Received 27 March 2009/ Accepted 12 May 2009

Bacillus thuringiensis subsp. israelensis produces a potent mosquitocidal protein, Cry4A. We have identified a 15-bp catabolite responsive element (cre), overlapping the –35 element of the cry4A promoter. Changing a guanine to adenine at position –49 in the promoter abolished glucose catabolite repression of cry4A and enhanced promoter activity two- to threefold. This cis regulatory element is essential for controlled toxin synthesis, vital to evolutionary success of B. thuringiensis subsp. israelensis.

* Corresponding author. Mailing address: International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India. Phone: 91-11-26741242. Fax: 91-11-26742316. E-mail: nirupama{at}icgeb.res.in

{triangledown} Published ahead of print on 22 May 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, July 2009, p. 4687-4692, Vol. 191, No. 14
0021-9193/09/$08.00+0     doi:10.1128/JB.00421-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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