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Journal of Bacteriology, August 2009, p. 5196-5204, Vol. 191, No. 16
0021-9193/09/$08.00+0     doi:10.1128/JB.00297-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of gshA, a Gene Encoding the Glutamate-Cysteine Ligase in the Halophilic Archaeon Haloferax volcanii{triangledown} ,{dagger}

Liron Malki, Michaela Yanku, Ilya Borovok, Gerald Cohen, Moshe Mevarech,* and Yair Aharonowitz*

Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel

Received 5 March 2009/ Accepted 2 June 2009

Halophilic archaea were found to contain in their cytoplasm millimolar concentrations of {gamma}-glutamylcysteine ({gamma}GC) instead of glutathione. Previous analysis of the genome sequence of the archaeon Halobacterium sp. strain NRC-1 has indicated the presence of a sequence homologous to sequences known to encode the glutamate-cysteine ligase GshA. We report here the identification of the gshA gene in the extremely halophilic archaeon Haloferax volcanii and show that H. volcanii gshA directs in vivo the synthesis and accumulation of {gamma}GC. We also show that the H. volcanii gene when expressed in an Escherichia coli strain lacking functional GshA is able to restore synthesis of glutathione.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv 69978, Israel. Phone for Yair Aharonowitz: 972-3-6409411. Fax: 972-3-6422245. E-mail: yaira{at}post.tau.ac.ilk. Phone for Moshe Mevarech: 972-3-6408715. Fax: 972-3-6409407. E-mail: mevarech{at}post.tau.ac.il

{triangledown} Published ahead of print on 12 June 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, August 2009, p. 5196-5204, Vol. 191, No. 16
0021-9193/09/$08.00+0     doi:10.1128/JB.00297-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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