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Journal of Bacteriology, August 2009, p. 5315-5319, Vol. 191, No. 16
0021-9193/09/$08.00+0     doi:10.1128/JB.00559-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Hydroxyl Radicals Are Involved in Cell Killing by the Bacterial Topoisomerase I Cleavage Complex{triangledown} ,{dagger}

I-Fen Liu, Thirunavukkarasu Annamalai, Jeanette H. Sutherland, and Yuk-Ching Tse-Dinh*

Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595

Received 27 April 2009/ Accepted 3 June 2009

Escherichia coli expressing SOS-inducing mutant topoisomerase I was utilized to demonstrate that covalent protein-DNA complex accumulation results in oxidative damage. Hydroxyl radicals were detected following mutant topoisomerase induction. The presence of the Fe2+ chelator 2,2'-dipyridyl and an iscS mutation affecting Fe-S cluster formation protect against topoisomerase I cleavage complex-mediated cell killing.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Basic Science Building, New York Medical College, Valhalla, NY 10595. Phone: (914) 594-4061. Fax: (914) 594-4058. E-mail: yuk-ching_tse-dinh{at}nymc.edu

{triangledown} Published ahead of print on 12 June 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, August 2009, p. 5315-5319, Vol. 191, No. 16
0021-9193/09/$08.00+0     doi:10.1128/JB.00559-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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