This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Haque, M. M.
Right arrow Articles by Tsuyumu, S.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haque, M. M.
Right arrow Articles by Tsuyumu, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2009, p. 5409-5418, Vol. 191, No. 17
0021-9193/09/$08.00+0     doi:10.1128/JB.00240-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

SlyA, a MarR Family Transcriptional Regulator, Is Essential for Virulence in Dickeya dadantii 3937{triangledown}

M. Manjurul Haque,1,§,{dagger} M. Shahinur Kabir,2,§ Luqman Qurata Aini,3 Hisae Hirata,1 and Shinji Tsuyumu1,2,3*

Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan,1 Institute for Genetic Research and Biotechnology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan,2 Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan3

Received 22 February 2009/ Accepted 6 June 2009

SlyA, a MarR family transcriptional regulator, controls an assortment of biological functions in several animal-pathogenic bacteria. In order to elucidate the functions of SlyA in the phytopathogen Dickeya dadantii (formerly Erwinia chrysanthemi) 3937, a slyA gene deletion mutant (denoted {Delta}slyA) was constructed. The mutant exhibited increased sensitivity to sodium hypochlorite, the cationic antimicrobial peptide polymyxin B, and oxidative stress. The mutant showed reduced production of pectate lyase and exopolysaccharide and an inability to form a pellicle. The mutant lacking a functional slyA gene showed a significantly reduced ability to cause maceration of potato tubers. Accordingly, the mutant exhibited significantly reduced bacterial growth and failed to hyperinduce pectate lyase production in planta. Introduction of a plasmid containing slyA into the {Delta}slyA mutant caused all of these phenotypes to recover to wild-type levels. These results suggest that SlyA plays an important role in virulence to plants by positively regulating the expression of multiple pathogenicity-related traits of D. dadantii 3937.

* Corresponding author. Mailing address: Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka Shi 422-8529, Japan. Phone and fax: 81-54-238-4823. E-mail: tsuyumu{at}agr.shizuoka.ac.jp

{triangledown} Published ahead of print on 19 June 2009.

§ M.M.H. and M.S.K. contributed equally to this work.

{dagger} Present address: Department of Bioenvironmental Science, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706, Bangladesh.

Journal of Bacteriology, September 2009, p. 5409-5418, Vol. 191, No. 17
0021-9193/09/$08.00+0     doi:10.1128/JB.00240-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»