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Journal of Bacteriology, September 2009, p. 5441-5445, Vol. 191, No. 17
0021-9193/09/$08.00+0     doi:10.1128/JB.00625-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Short-Chain Chromate Ion Transporter Proteins from Bacillus subtilis Confer Chromate Resistance in Escherichia coli{triangledown}

Amada Díaz-Magaña,1 Esther Aguilar-Barajas,1 Rafael Moreno-Sánchez,2 Martha I. Ramírez-Díaz,1 Héctor Riveros-Rosas,3 Eréndira Vargas,1 and Carlos Cervantes1*

Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana, Morelia, Michoacán,1 Departamento de Bioquímica, Instituto Nacional de Cardiología, Mexico City,2 Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico3

Received 12 May 2009/ Accepted 23 June 2009

Tandem paired genes encoding putative short-chain monodomain protein members of the chromate ion transporter (CHR) superfamily (ywrB and ywrA) were cloned from genomic DNA of Bacillus subtilis strain 168. The transcription of the paired genes, renamed chr3N and chr3C, respectively, was shown to occur via a bicistronic mRNA generated from a promoter upstream of the chr3N gene. The chr3N and chr3C genes conferred chromate resistance when expressed in Escherichia coli strain W3110. The cloned chr3N gene alone did not confer chromate resistance on E. coli, suggesting that both chr3N and chr3C genes are required for function. E. coli cells expressing paired chr3N and chr3C genes demonstrated diminished uptake of chromate compared to that by a vector-only control strain. These results suggest that short-chain CHR proteins form heterodimer transporters which efflux chromate ions from the cytoplasm.

* Corresponding author. Mailing address: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana, Edificio B-3, Ciudad Universitaria, 58030 Morelia, Mich., Mexico. Phone and fax: 52 (443) 326-5788. E-mail: cvega1999{at}yahoo.com

{triangledown} Published ahead of print on 6 July 2009.

Journal of Bacteriology, September 2009, p. 5441-5445, Vol. 191, No. 17
0021-9193/09/$08.00+0     doi:10.1128/JB.00625-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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