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Journal of Bacteriology, September 2009, p. 5480-5488, Vol. 191, No. 17
0021-9193/09/$08.00+0     doi:10.1128/JB.00640-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum{triangledown}

Jung-Won Youn,1,{dagger} Elena Jolkver,2,{dagger} Reinhard Krämer,2 Kay Marin,2* and Volker F. Wendisch1*

Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Muenster, Germany,1 Institute of Biochemistry, Cologne University, Cologne, Germany2

Received 14 May 2009/ Accepted 24 June 2009

Transporters of the dicarboxylate amino acid-cation symporter family often mediate uptake of C4-dicarboxylates, such as succinate or L-malate, in bacteria. A member of this family, dicarboxylate transporter A (DctA) from Corynebacterium glutamicum, was characterized to catalyze uptake of the C4-dicarboxylates succinate, fumarate, and L-malate, which was inhibited by oxaloacetate, 2-oxoglutarate, and glyoxylate. DctA activity was not affected by sodium availability but was dependent on the electrochemical proton potential. Efficient growth of C. glutamicum in minimal medium with succinate, fumarate, or L-malate as the sole carbon source required high dctA expression levels due either to a promoter-up mutation identified in a spontaneous mutant or to ectopic overexpression. Mutant analysis indicated that DctA and DccT, a C4-dicarboxylate divalent anion/sodium symporter-type transporter, are the only transporters for succinate, fumarate, and L-malate in C. glutamicum.

* Corresponding author. Mailing address for V. F. Wendisch: Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Corrensstr. 3, D-48149 Muenster, Germany. Phone: 49-251-833 9827. Fax: 49-251-833 8388. E-mail: wendisch{at}uni-muenster.de. Mailing address for K. Marin: Institute of Biochemistry, Cologne University, Cologne, Germany. Phone: 49-221-470 6476. Fax: 49-221-470 5091. E-mail: kay.marin{at}uni-koeln.de

{triangledown} Published ahead of print on 6 July 2009.

{dagger} J.-W.Y. and E.J. contributed equally to this work.

Journal of Bacteriology, September 2009, p. 5480-5488, Vol. 191, No. 17
0021-9193/09/$08.00+0     doi:10.1128/JB.00640-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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