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Journal of Bacteriology, September 2009, p. 5577-5583, Vol. 191, No. 18
0021-9193/09/$08.00+0     doi:10.1128/JB.00493-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparisons between Geographically Diverse Samples of Carried Staphylococcus aureus{triangledown}

Raymond Ruimy,1,2* Laurence Armand-Lefevre,1,2,{dagger} Francois Barbier,1,2,{dagger} Etienne Ruppé,1,2,{dagger} Radu Cocojaru,1,3,{dagger} Yasmine Mesli,1,4,{dagger} Aminata Maiga,1,5 Mokhtar Benkalfat,6 Samia Benchouk,4 Hafida Hassaine,7 Jean-Baptiste Dufourcq,8 Chhor Nareth,8 Jean-Louis Sarthou,9 Antoine Andremont,1,2 and Edward J. Feil10

Laboratoire Associé Centre National de Référence Résistance dans les Flores Commensales, Hôpital Bichat-Claude Bernard (AP-HP), 75018 Paris, France,1 EA 3469 University Paris 7-Diderot, Paris, France,2 National Center for Preventive Medicine, Chisinau, Moldova,3 Service des Maladies Infectieuses, Hôpital Damerdji Tidjani, Tlemcen, Algeria,4 Laboratoire de Biologie Médicale et d'Hygiène, CHU point G, Bamako Mali,5 Service de Chirurgie Viscérale, Hôpital Damerdji Tidjani, Tlemcen, Algeria,6 Faculté des Sciences, Tlemcen, Algeria,7 Service des Urgences, Hôpital Calmette, Phnom Penh, Cambodia,8 Institut Pasteur du Cambodge, Phnom Penh, Cambodia,9 Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom,10

Received 9 April 2009/ Accepted 1 July 2009

Approximately one-third of the human population is asymptomatically colonized by Staphylococcus aureus. However, much of the global diversity within the carriage populations remains uncharacterized, and it is unclear to what degree the variation is geographically partitioned. We isolated 300 carriage isolates from 1,531 adults contemporaneously in four countries: France, Algeria, Moldova, and Cambodia. All strains were characterized by multilocus sequence typing. Six clonal complexes (CCs) were present in all four samples (CC30, -45, -121, -15, -5, and -8). Analyses based on the genotype frequencies revealed the French and Algerian samples to be most similar and the Cambodian sample to be most distinct. While this pattern is consistent with likely rates of human migration and geographic distance, stochastic clonal expansion also contributes to regional differences. Phylogenetic analysis revealed a highly divergent and uncharacterized genotype (ST1223) within Cambodia. This lineage is related to CC75, which has previously been observed only in remote aboriginal populations in northern Australia.

* Corresponding author. Mailing address: Hospital Group Bichat-Claude Bernard, Laboratoire de Bactériologie, 46, Rue Henri-Huchard, 75018 Paris, France. Phone: 33 1 40 25 85 05. Fax: 33 1 40 25 85 81. E-mail: raymond.ruimy{at}bch.ap-hop-paris.fr

{triangledown} Published ahead of print on 10 July 2009.

{dagger} These authors contributed equally to this work.

Journal of Bacteriology, September 2009, p. 5577-5583, Vol. 191, No. 18
0021-9193/09/$08.00+0     doi:10.1128/JB.00493-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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