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Journal of Bacteriology, September 2009, p. 5628-5633, Vol. 191, No. 18
0021-9193/09/$08.00+0     doi:10.1128/JB.00510-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Mycobacterium tuberculosis Sigma Factor {sigma}B Is Required for Full Response to Cell Envelope Stress and Hypoxia In Vitro, but It Is Dispensable for In Vivo Growth{triangledown} ,{ddagger}

P. A. Fontán,1 M. I. Voskuil,2,3 M. Gomez,1 D. Tan,4,{dagger} M. Pardini,4 R. Manganelli,5 L. Fattorini,4 G. K. Schoolnik,3 and I. Smith1*

PHRI Center of NJMS-UMDNJ, 225 Warren Street, Newark, New Jersey 07103-3535,1 Department of Microbiology, School of Medicine, University of Colorado Denver, P18-9115, 12800 East 19th Avenue, P.O. Box 6511, Aurora, Colorado 80045,2 Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, 300 Pasteur Dr., B241 MC 5428, Stanford, California 94305,3 Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy,4 Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Medical School, Via A. Gabelli 63, 35121 Padova, Italy5

Received 14 April 2009/ Accepted 22 June 2009

The numerous sigma ({sigma}) factors present in Mycobacterium tuberculosis are indicative of the adaptability of this pathogen to different environmental conditions. In this report, we describe the M. tuberculosis {sigma}B regulon and the phenotypes of an M. tuberculosis sigB mutant strain exposed to cell envelope stress, oxidative stress, and hypoxia. The sigB mutant was especially defective in survival under hypoxic conditions in vitro, but it was not attenuated for growth in THP-1 cells or during mouse and guinea pig infection.

* Corresponding author. Mailing address: PHRI TB Center, 225 Warren Street, Room W210W, Newark, NJ 07103-3535. Phone: (973) 854-3260. Fax: (973) 854-3101. E-mail: smithis{at}umdnj.edu

{triangledown} Published ahead of print on 10 July 2009.

{ddagger} Supplemental material for this article may be found at http://jb.asm.org/.

{dagger} Present address: National Institute for the Control of Pharmaceutical and Biological Products 2#, Tiantan Xili, Beijing, China 100050.

Journal of Bacteriology, September 2009, p. 5628-5633, Vol. 191, No. 18
0021-9193/09/$08.00+0     doi:10.1128/JB.00510-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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