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Journal of Bacteriology, September 2009, p. 5641-5647, Vol. 191, No. 18
0021-9193/09/$08.00+0     doi:10.1128/JB.00448-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

An Alternative Route for Recycling of N-Acetylglucosamine from Peptidoglycan Involves the N-Acetylglucosamine Phosphotransferase System in Escherichia coli{triangledown}

Jacqueline Plumbridge*

Institut de Biologie Physico-Chimique, (UPR9073-CNRS) 13, rue Pierre et Marie Curie, 75005 Paris, France

Received 1 April 2009/ Accepted 9 July 2009

A set of enzymes dedicated to recycling of the amino sugar components of peptidoglycan has previously been identified in Escherichia coli. The complete pathway includes the nagA-encoded enzyme, N-acetylglucosamine-6-phosphate (GlcNAc6P) deacetylase, of the catabolic pathway for use of N-acetylglucosamine (GlcNAc). Mutations in nagA result in accumulation of millimolar concentrations of GlcNAc6P, presumably by preventing peptidoglycan recycling. Mutations in the genes encoding the key enzymes upstream of nagA in the dedicated recycling pathway (ampG, nagZ, nagK, murQ, and anmK), which were expected to interrupt the recycling process, reduced but did not eliminate accumulation of GlcNAc6P. A mutation in the nagE gene of the GlcNAc phosphotransferase system (PTS) was found to reduce by 50% the amount of GlcNAc6P which accumulated in a nagA strain and, together with mutations in the dedicated recycling pathway, eliminated all the GlcNAc6P accumulation. This shows that the nagE-encoded PTS transporter makes an important contribution to the recycling of peptidoglycan. The manXYZ-encoded PTS transporter makes a minor contribution to the formation of cytoplasmic GlcNAc6P but appears to have a more important role in secretion of GlcNAc and/or GlcNAc6P from the cytoplasm.

* Mailing address: Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, 75005 Paris, France. Phone: 33 (0)1 58 41 51 52. Fax: 33 (0)1 58 41 50 20. E-mail: Jackie.Plumbridge{at}ibpc.fr

{triangledown} Published ahead of print on 17 July 2009.

Journal of Bacteriology, September 2009, p. 5641-5647, Vol. 191, No. 18
0021-9193/09/$08.00+0     doi:10.1128/JB.00448-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Alvarez-Anorve, L. I., Bustos-Jaimes, I., Calcagno, M. L., Plumbridge, J. (2009). Allosteric Regulation of Glucosamine-6-Phosphate Deaminase (NagB) and Growth of Escherichia coli on Glucosamine. J. Bacteriol. 191: 6401-6407 [Abstract] [Full Text]  


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