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Journal of Bacteriology, September 2009, p. 5873-5876, Vol. 191, No. 18
0021-9193/09/$08.00+0     doi:10.1128/JB.01206-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

DNA Binding Proteins of the Filamentous Phages CTX{phi} and VGJ{phi} of Vibrio cholerae{triangledown}

Alina Falero,* Andy Caballero, Beatriz Ferrán, Yovanny Izquierdo, Rafael Fando, and Javier Campos*

Department of Molecular Biology, National Center for Scientific Research, P.O. Box 6414, Ave. 25 y 158, Cubanacán, Playa, Ciudad de la Habana, Cuba

Received 27 August 2008/ Accepted 7 July 2009

The native product of open reading frame 112 (orf112) and a recombinant variant of the RstB protein, encoded by Vibrio cholerae pathogen-specific bacteriophages VGJ{phi} and CTX{phi}, respectively, were purified to more than 90% homogeneity. Orf112 protein was shown to specifically bind single-stranded genomic DNA of VGJ{phi}; however, RstB protein unexpectedly bound double-stranded DNA in addition to the single-stranded genomic DNA. The DNA binding properties of these proteins may explain their requirement for the rolling circle replication of the respective phages and RstB's requirement for single-stranded-DNA chromosomal integration of CTX{phi} phage dependent on XerCD recombinases.

* Corresponding author. Mailing address: Department of Molecular Biology, National Center for Scientific Research, P.O. Box 6414, Ave. 25 y 158, Cubanacán, Playa, Ciudad de la Habana, Cuba. Phone for Alina Falero: 537 2085236. Fax: 537 2080497. E-mail: alina.falero{at}cnic.edu.cu. Phone for Javier Campos: 537 2086548. Fax: 537 2080497. E-mail: javier.campos{at}cnic.edu.cu

{triangledown} Published ahead of print on 17 July 2009.

Journal of Bacteriology, September 2009, p. 5873-5876, Vol. 191, No. 18
0021-9193/09/$08.00+0     doi:10.1128/JB.01206-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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