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Journal of Bacteriology, October 2009, p. 6178-6185, Vol. 191, No. 19
0021-9193/09/$08.00+0     doi:10.1128/JB.00723-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of the FtsZ-Interacting Septal Proteins SepF and Ftn6 in the Spherical-Celled Cyanobacterium Synechocystis Strain PCC 6803{triangledown}

Martial Marbouty,1 Cyril Saguez,1 Corinne Cassier-Chauvat,1,2* and Franck Chauvat1*

CEA, iBiTec-S, SBIGeM, LBI, Bat 142 CEA-Saclay, F-91191 Gif sur Yvette CEDEX, France,1 CNRS, URA 2096, F-91191 Gif sur Yvette CEDEX, France2

Received 4 June 2009/ Accepted 24 July 2009

Assembly of the tubulin-like cytoskeletal protein FtsZ into a ring structure at midcell establishes the location of the nascent division sites in prokaryotes. However, it is not yet known how the assembly and contraction of the Z ring are regulated, especially in cyanobacteria, the environmentally crucial organisms for which only one FtsZ partner protein, ZipN, has been described so far. Here, we characterized SepF and Ftn6, two novel septal proteins, in the spherical-celled strain Synechocystis PCC 6803. Both proteins were found to be indispensable to Synechocystis sp. strain PCC 6803. The depletion of both SepF and Ftn6 resulted in delayed cytokinesis and the generation of giant cells but did not prevent FtsZ polymerization, as shown by the visualization of green fluorescent protein (GFP)-tagged FtsZ polymers. These GFP-tagged Z-ring-like structures often appeared to be abnormal, because these reporter cells respond to the depletion of either SepF or Ftn6 with an increased abundance of total, natural, and GFP-tagged FtsZ proteins. In agreement with their septal localization, we found that both SepF and Ftn6 interact physically with FtsZ. Finally, we showed that SepF, but not Ftn6, stimulates the formation and/or stability of FtsZ polymers in vitro.

* Corresponding author. Mailing address for Franck Chauvat: CEA, iBiTec-S, SBIGeM, LBI, Bat 142 CEA-Saclay, F-91191 Gif sur Yvette CEDEX, France. Phone: (33) 1 69 08 78 11. Fax: (33) 1 69 08 47 12. E-mail: franck.chauvat{at}cea.fr. Mailing address for Corinne Cassier-Chauvat: CEA, iBiTec-S, SBIGeM, LBI, Bat 142 CEA-Saclay, F-91191 Gif sur Yvette CEDEX, France. Phone: (33) 1 69 08 35 74. Fax: (33) 1 69 08 47 12. E-mail: corinne-cassier.chauvat{at}cea.fr

{triangledown} Published ahead of print on 31 July 2009.

Journal of Bacteriology, October 2009, p. 6178-6185, Vol. 191, No. 19
0021-9193/09/$08.00+0     doi:10.1128/JB.00723-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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