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Journal of Bacteriology, January 2009, p. 555-562, Vol. 191, No. 2
0021-9193/09/$08.00+0     doi:10.1128/JB.00216-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of the Mycobacterial NER System Reveals Novel Functions of the uvrD1 Helicase{triangledown}

Carolin Güthlein,1 Roger M. Wanner,1 Peter Sander,1,2 Elaine O. Davis,3 Martin Bosshard,1 Josef Jiricny,4 Erik C. Böttger,1,2* and Burkhard Springer1,2,{dagger}

Institut für Medizinische Mikrobiologie, University of Zurich, Gloriastrasse 30/32, CH-8006, Zurich, Switzerland,1 Nationales Zentrum für Mykobakterien, Gloriastrasse 30, CH-8006 Zurich, Switzerland,2 Division of Mycobacterial Research, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom,3 Institute of Molecular Cancer Research, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, and Department of Biology, Swiss Federal Institutes of Technology (ETH), CH-8093 Zurich, Switzerland4

Received 12 February 2008/ Accepted 26 October 2008

In this study, we investigated the role of the nucleotide excision repair (NER) pathway in mycobacterial DNA repair. Mycobacterium smegmatis lacking the NER excinuclease component uvrB or the helicase uvrD1 gene and a double knockout lacking both genes were constructed, and their sensitivities to a series of DNA-damaging agents were analyzed. As anticipated, the mycobacterial NER system was shown to be involved in the processing of bulky DNA adducts and interstrand cross-links. In addition, it could be shown to exert a protective effect against oxidizing and nitrosating agents. Interestingly, inactivation of uvrB and uvrD1 significantly increased marker integration frequencies in gene conversion assays. This implies that in mycobacteria (which lack the postreplicative mismatch repair system) NER, and particularly the UvrD1 helicase, is involved in the processing of a subset of recombination-associated mismatches.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie, Universität Zürich, Gloriastrasse 30/32, 8006 Zürich, Switzerland. Phone: 41 44 634 2660. Fax: 41 44 634 4906. E-mail: boettger{at}imm.uzh.ch

{triangledown} Published ahead of print on 14 November 2008.

{dagger} Present address: Institut für Medizinische Mikrobiologie und Hygiene, Agentur für Gesundheit und Ernährungssicherheit, Beethovenstrasse 6, 8010 Graz, Austria.

Journal of Bacteriology, January 2009, p. 555-562, Vol. 191, No. 2
0021-9193/09/$08.00+0     doi:10.1128/JB.00216-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wanner, R. M., Castor, D., Guthlein, C., Bottger, E. C., Springer, B., Jiricny, J. (2009). The Uracil DNA Glycosylase UdgB of Mycobacterium smegmatis Protects the Organism from the Mutagenic Effects of Cytosine and Adenine Deamination. J. Bacteriol. 191: 6312-6319 [Abstract] [Full Text]  


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