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Journal of Bacteriology, November 2009, p. 6632-6642, Vol. 191, No. 21
0021-9193/09/$08.00+0     doi:10.1128/JB.00708-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

PhoB Regulates Motility, Biofilms, and Cyclic di-GMP in Vibrio cholerae{triangledown}

Jason T. Pratt,2 EmilyKate McDonough,2 and Andrew Camilli1,2*

Howard Hughes Medical Institute,1 Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 021112

Received 31 May 2009/ Accepted 25 August 2009

Signaling through the second messenger cyclic di-GMP (c-di-GMP) is central to the life cycle of Vibrio cholerae. However, relatively little is known about the signaling mechanism, including the specific external stimuli that regulate c-di-GMP concentration. Here, we show that the phosphate responsive regulator PhoB regulates an operon, acgAB, which encodes c-di-GMP metabolic enzymes. We show that induction of acgAB by PhoB positively regulates V. cholerae motility in vitro and that PhoB regulates expression of acgAB at late stages during V. cholerae infection in the infant mouse small intestine. These data support a model whereby PhoB becomes activated at a late stage of infection in preparation for dissemination of V. cholerae to the aquatic environment and suggest that the concentration of exogenous phosphate may become limited at late stages of infection.

* Corresponding author. Mailing address: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111. Phone: (617) 636-2144. Fax: (617) 636-2175. E-mail: andrew.camilli{at}tufts.edu

{triangledown} Published ahead of print on 4 September 2009.

Journal of Bacteriology, November 2009, p. 6632-6642, Vol. 191, No. 21
0021-9193/09/$08.00+0     doi:10.1128/JB.00708-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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