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Journal of Bacteriology, November 2009, p. 6773-6777, Vol. 191, No. 21
0021-9193/09/$08.00+0     doi:10.1128/JB.00832-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

A Novel Insertion Sequence Derepresses Efflux Pump Expression and Preadapts Pseudomonas putida S12 for Extreme Solvent Stress{triangledown}

Xu Sun and Jonathan J. Dennis*

Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E9

Received 25 June 2009/ Accepted 24 August 2009

A multidrug efflux pump, SrpABC, plays a key role in Pseudomonas putida S12 tolerance to toxic organic solvents. SrpRS are putative regulators of the SrpABC efflux pump encoded upstream of the srpABC structural genes, and previous studies suggest that SrpS is a repressor of SrpABC expression. An S12 isolate able to withstand extreme solvent stress carries a novel insertion sequence, ISPpu21, interrupting srpS. This insertion preadapts S12 to extreme solvent conditions through constitutive SrpABC expression.

* Corresponding author. Mailing address: Department of Biological Sciences, M354 Biological Sciences Bldg., University of Alberta, Edmonton, AB, Canada T6G 2E9. Phone: (780) 492-2529. Fax: (780) 492-9234. E-mail: jon.dennis{at}ualberta.ca

{triangledown} Published ahead of print on 28 August 2009.

Journal of Bacteriology, November 2009, p. 6773-6777, Vol. 191, No. 21
0021-9193/09/$08.00+0     doi:10.1128/JB.00832-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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