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Journal of Bacteriology, November 2009, p. 6796-6803, Vol. 191, No. 22
0021-9193/09/$08.00+0     doi:10.1128/JB.00798-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Scavenging of Cytosolic Octanoic Acid by Mutant LplA Lipoate Ligases Allows Growth of Escherichia coli Strains Lacking the LipB Octanoyltransferase of Lipoic Acid Synthesis{triangledown}

Fatemah A. M. Hermes1 and John E. Cronan1,2*

Departments of Microbiology,1 Biochemistry, University of Illinois, Urbana, Illinois 618012

Received 18 June 2009/ Accepted 4 August 2009

The LipB octanoyltransferase catalyzes the first step of lipoic acid synthesis in Escherichia coli, transfer of the octanoyl moiety from octanoyl-acyl carrier protein to the lipoyl domains of the E2 subunits of the 2-oxoacid dehydrogenases of aerobic metabolism. Strains containing null mutations in lipB are auxotrophic for either lipoic acid or octanoic acid. We report the isolation of two spontaneously arising mutant strains that allow growth of lipB strains on glucose minimal medium; we determined that suppression was caused by single missense mutations within the coding sequence of the gene (lplA) that encodes lipoate-protein ligase. The LplA proteins encoded by the mutant genes have reduced Km values for free octanoic acid and thus are able to scavenge cytosolic octanoic acid for octanoylation of lipoyl domains.

* Corresponding author. Mailing address: Department of Microbiology, University of Illinois, B103 CLSL, 601 S. Goodwin Ave., Urbana, IL 61801. Phone: (217) 333-7919. Fax: (217) 244-6697. E-mail: j-cronan{at}life.uiuc.edu

{triangledown} Published ahead of print on 14 August 2009.

Journal of Bacteriology, November 2009, p. 6796-6803, Vol. 191, No. 22
0021-9193/09/$08.00+0     doi:10.1128/JB.00798-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Related articles in JB:

Opening a New Path to Lipoic Acid
Charles O. Rock
JB 2009 191: 6782-6784. [Full Text]  



This article has been cited by other articles:

  • Rock, C. O. (2009). Opening a New Path to Lipoic Acid. J. Bacteriol. 191: 6782-6784 [Full Text]  


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