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Journal of Bacteriology, December 2009, p. 7367-7371, Vol. 191, No. 23
0021-9193/09/$08.00+0     doi:10.1128/JB.01081-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

ScoC Regulates Bacilysin Production at the Transcription Level in Bacillus subtilis{triangledown}

Takashi Inaoka,* Guojun Wang, and Kozo Ochi

Microbial Function Laboratory, National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan

Received 17 August 2009/ Accepted 22 September 2009

Bacillus subtilis mutants with high expression of the bacilysin operon ywfBCDEFG were isolated. Comparative genome sequencing analysis revealed that all of these mutants have a mutation in the scoC gene. The disruption of scoC by genetic engineering also resulted in increased expression of ywfBCDEFG. Primer extension and gel mobility shift analyses showed that the ScoC protein binds directly to the promoter region of ywfBCDEFG. Our results indicate that the transition state regulator ScoC, together with CodY and AbrB, negatively regulates bacilysin production in B. subtilis.

* Corresponding author. Mailing address: National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. Phone: 81-29-838-8124. Fax: 81-29-838-7996. E-mail: tina2672{at}affrc.go.jp

{triangledown} Published ahead of print on 2 October 2009.

Journal of Bacteriology, December 2009, p. 7367-7371, Vol. 191, No. 23
0021-9193/09/$08.00+0     doi:10.1128/JB.01081-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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