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Journal of Bacteriology, February 2009, p. 1343-1348, Vol. 191, No. 4
0021-9193/09/$08.00+0     doi:10.1128/JB.01074-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Leucine-Responsive Regulatory Protein, Lrp, Modulates Microcin J25 Intrinsic Resistance in Escherichia coli by Regulating Expression of the YojI Microcin Exporter{triangledown}

Sergio B. Socías, Paula A. Vincent, and Raúl A. Salomón*

Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas—Universidad Nacional de Tucumán), and Instituto de Química Biológica Dr. Bernabé Bloj, Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina

Received 1 August 2008/ Accepted 1 December 2008

Many Escherichia coli K-12 strains display an intrinsic resistance to the peptide antibiotic microcin J25. In this study, we present results showing that the leucine-responsive regulatory protein, Lrp, is involved in this phenotype by acting as a positive regulator of YojI, a chromosomally encoded efflux pump which expels microcin out of cells. Exogenous leucine antagonizes the effect of Lrp, leading to a diminished expression of the pump and an increased susceptibility to microcin J25.

* Corresponding author. Mailing address: Departamento de Bioquímica de la Nutrición, INSIBIO, Chacabuco 461, 4000 San Miguel de Tucumán, Argentina. Phone and fax: 54 381 4248921. E-mail: salomon{at}fbqf.unt.edu.ar

{triangledown} Published ahead of print on 12 December 2008.

Journal of Bacteriology, February 2009, p. 1343-1348, Vol. 191, No. 4
0021-9193/09/$08.00+0     doi:10.1128/JB.01074-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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