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Journal of Bacteriology, March 2009, p. 1703-1715, Vol. 191, No. 5
0021-9193/09/$08.00+0     doi:10.1128/JB.01021-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Essential Role of the SycP Chaperone in Type III Secretion of the YspP Effector{triangledown} ,§

Hiroyuki Matsumoto and Glenn M. Young*

Department of Food Science and Technology, University of California, Davis, California 95616

Received 24 July 2008/ Accepted 20 December 2008

The Ysa type III secretion (T3S) system enhances gastrointestinal infection by Yersinia enterocolitica bv. 1B. One effector protein targeted into host cells is YspP, a protein tyrosine phosphatase. It was determined in this study that the secretion of YspP requires a chaperone, SycP. Genetic analysis showed that deletion of sycP completely abolished the secretion of YspP without affecting the secretion of other Ysps by the Ysa T3S system. Analysis of the secretion and translocation signals of YspP defined the first 73 amino acids to form the minimal region of YspP necessary to promote secretion and translocation by the Ysa T3S system. Function of the YspP secretion/translocation signals was dependent on SycP. Curiously, when YspP was constitutively expressed in Y. enterocolitica bv. 1B, it was recognized and secreted by the Ysc T3S system and the flagellar T3S system. In these cases, the first 21 amino acids were sufficient to promote secretion, and while SycP did enhance secretion, it was not essential. However, neither the Ysc T3S system nor the flagellar T3S system translocated YspP into mammalian cells. This supports a model where SycP confers secretion/translocation specificities for YspP by the Ysa T3S system. A series of biochemical approaches further established that SycP specifically interacts with YspP and protected YspP degradation in the cell prior to secretion. Collectively, the evidence suggests that YspP secretion by the Ysa T3S system is a posttranslational event.

* Corresponding author. Mailing address: University of California, One Shields Avenue, FS&T, RMI North Laboratory, Davis, CA 95616. Phone: (530) 754-5292. Fax: (530) 752-4759. E-mail: gmyoung{at}ucdavis.edu

{triangledown} Published ahead of print on 29 December 2008.

§ Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, March 2009, p. 1703-1715, Vol. 191, No. 5
0021-9193/09/$08.00+0     doi:10.1128/JB.01021-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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