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Journal of Bacteriology, March 2009, p. 1722-1724, Vol. 191, No. 5
0021-9193/09/$08.00+0     doi:10.1128/JB.01573-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Conditional Essentiality of the csrA Gene in Escherichia coli{triangledown}

Johan Timmermans and Laurence Van Melderen*

Laboratoire de Génétique et de Physiologie Bactérienne, Institut de Biologie et de Médecine Moléculaires, Faculté des Sciences, Université Libre de Bruxelles, 12 rue des Professeurs Jeener et Brachet, B:6041 Gosselies, Belgium

Received 5 November 2008/ Accepted 10 December 2008

CsrA is a global posttranscriptional regulator of numerous physiological processes, such as glycogenesis and glycolysis. Here, we show that the csrA gene of Escherichia coli is essential for growth on LB and on synthetic medium containing glycolytic carbon sources. However, csrA is not necessary for growth on synthetic medium containing pyruvate, showing that the Krebs cycle is functional in the csrA::cat deletion mutant. Deletion of the glgCAP operon in the csrA::cat mutant restored the ability to grow on LB and on synthetic medium containing glycolytic carbon sources, showing that growth inhibition is due to an excess of glycogen synthesis.

* Corresponding author. Mailing address: Laboratoire de Génétique et de Physiologie Bactérienne, Institut de Biologie et de Médecine Moléculaires, Faculté des Sciences, Université Libre de Bruxelles, 12 rue des Professeurs Jeener et Brachet, B:6041 Gosselies, Belgium. Phone: 32 2 650 97 78. Fax: 32 2 650 97 70. E-mail: lvmelder{at}ulb.ac.be

{triangledown} Published ahead of print on 19 December 2008.

Journal of Bacteriology, March 2009, p. 1722-1724, Vol. 191, No. 5
0021-9193/09/$08.00+0     doi:10.1128/JB.01573-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jin, Y., Watt, R. M., Danchin, A., Huang, J.-d. (2009). Use of a Riboswitch-controlled Conditional Hypomorphic Mutation to Uncover a Role for the Essential csrA Gene in Bacterial Autoaggregation. J. Biol. Chem. 284: 28738-28745 [Abstract] [Full Text]  


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