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Journal of Bacteriology, March 2009, p. 1838-1846, Vol. 191, No. 6
0021-9193/09/$08.00+0     doi:10.1128/JB.01388-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Regulation of Expression of the Region 3 Promoter of the Escherichia coli K5 Capsule Gene Cluster Involves H-NS, SlyA, and a Large 5' Untranslated Region{triangledown}

Peng Xue, David Corbett, Marie Goldrick, Clare Naylor, and Ian S. Roberts*

Faculty of Life Sciences, University of Manchester, Manchester M139PT, United Kingdom

Received 3 October 2008/ Accepted 21 December 2008

Escherichia coli group 2 capsule gene clusters are temperature regulated, being expressed at 37°C but not at 20°C. Expression is regulated at the level of transcription by two convergent promoters, PR1 and PR3. In this paper, we show that regulation of transcription from PR3 involves a number of novel features including H-NS, SlyA, and a large 741-bp 5' untranslated region (UTR). H-NS represses transcription from PR3 at 20°C and binds both 5' and 3' of the transcription start site. The 3' downstream regulatory element (DRE) was essential for temperature-dependent H-NS repression. At 37°C, SlyA activates transcription independent of H-NS but maximal transcription requires H-NS. The UTR is present between the transcription start site and the first gene in the operon, kpsM. We demonstrate that the UTR, as well as containing the H-NS DRE, functions to moderate the extent of transcription that reaches kpsM and allows the binding of antitermination factor RfaH.

* Corresponding author. Mailing address: Faculty of Life Sciences, University of Manchester, Michael Smith Building, Dover Street, Manchester M13 9PT, United Kingdom. Phone: 0044-(0)161 275 5753. Fax: 0044-(0)161 275 5656. E-mail: i.s.roberts{at}manchester.ac.uk

{triangledown} Published ahead of print on 29 December 2008.

Journal of Bacteriology, March 2009, p. 1838-1846, Vol. 191, No. 6
0021-9193/09/$08.00+0     doi:10.1128/JB.01388-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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