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Journal of Bacteriology, March 2009, p. 1982-1985, Vol. 191, No. 6
0021-9193/09/$08.00+0     doi:10.1128/JB.01012-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Regions on Gli349 and Gli521 Protein Molecules Directly Involved in Movements of Mycoplasma mobile Gliding Machinery, Suggested by Use of Inhibitory Antibodies and Mutants {triangledown} ,{dagger}

Atsuko Uenoyama, Shintaro Seto,{ddagger} Daisuke Nakane, and Makoto Miyata*

Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan

Received 23 July 2008/ Accepted 18 December 2008

Mycoplasma mobile glides on solid surfaces by use of a unique mechanism that involves two large proteins, Gli349 and Gli521. Here we isolated and analyzed two antibodies and three mutants that modified mycoplasma gliding. Mapping of the target points of antibodies and mutations currently available suggested that a 301-amino-acid region on the whole 3,138-amino-acid sequence, a C-terminal region of Gli349, and an N-terminal region of Gli521 are directly involved in the movements of the gliding machinery.

* Corresponding author. Mailing address: Department of Biology, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. Phone: 81 (6) 6605 3157. Fax: 81 (6) 6605 3158. E-mail: miyata{at}sci.osaka-cu.ac.jp

{triangledown} Published ahead of print on 5 January 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Department of Infectious Diseases, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan.

Journal of Bacteriology, March 2009, p. 1982-1985, Vol. 191, No. 6
0021-9193/09/$08.00+0     doi:10.1128/JB.01012-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakane, D., Miyata, M. (2009). Cytoskeletal Asymmetrical Dumbbell Structure of a Gliding Mycoplasma, Mycoplasma gallisepticum, Revealed by Negative-Staining Electron Microscopy. J. Bacteriol. 191: 3256-3264 [Abstract] [Full Text]  


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