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Journal of Bacteriology, April 2009, p. 2228-2236, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01395-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genetics and Proteomics of Aeromonas salmonicida Lipopolysaccharide Core Biosynthesis{triangledown} ,{dagger}

Natalia Jimenez,1 Anna Lacasta,1 Silvia Vilches,1 Mercé Reyes,1 Judit Vazquez,1 Eleonora Aquillini,2 Susana Merino,1 Miguel Regué,2 and Juan M. Tomás1*

Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08071 Barcelona,1 Departamento de Microbiología y Parasitología Sanitarias, Facultad de Farmacia, Universidad de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain2

Received 6 October 2008/ Accepted 2 January 2009

Comparison between the lipopolysaccharide (LPS) core structures of Aeromonas salmonicida subsp. salmonicida A450 and Aeromonas hydrophila AH-3 shows great similarity in the inner LPS core and part of the outer LPS core but some differences in the distal part of the outer LPS core (residues LD-Hep, D-Gal, and D-GalNAc). The three genomic regions encoding LPS core biosynthetic genes in A. salmonicida A450, of which regions 2 and 3 have genes identical to those of A. hydrophila AH-3, were fully sequenced. A. salmonicida A450 region 1 showed seven genes: three identical to those of A. hydrophila AH-3, three similar but not identical to those of A. hydrophila AH-3, and one without any homology to any well-characterized gene. A. salmonicida A450 mutants with alterations in the genes that were not identical to those of A. hydrophila AH-3 were constructed, and their LPS core structures were fully elucidated. At the same time, all the A. salmonicida A450 genes identical to those of A. hydrophila AH-3 were used to complement the previously obtained A. hydrophila AH-3 mutants for each of these genes. Combining the gene sequence and complementation test data with the structural data and phenotypic characterization of the mutant LPSs enabled a presumptive assignment of all LPS core biosynthesis gene functions in A. salmonicida A450. Furthermore, hybridization studies with internal probes for the A. salmonicida-specific genes using different A. salmonicida strains (strains of different subspecies or atypical strains) showed a unique or prevalent LPS core type, which is the one fully characterized for A. salmonicida A450.

* Corresponding author. Mailing address: Departamento Microbiología, Facultad Biología, Universidad Barcelona, Diagonal 645, 08071 Barcelona, Spain. Phone: 34-93-4021486. Fax: 34-93-4039047. E-mail: jtomas{at}ub.edu

{triangledown} Published ahead of print on 16 January 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, April 2009, p. 2228-2236, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01395-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jimenez, N., Vilches, S., Lacasta, A., Regue, M., Merino, S., Tomas, J. M. (2009). A Bifunctional Enzyme in a Single Gene Catalyzes the Incorporation of GlcN into the Aeromonas Core Lipopolysaccharide. J. Biol. Chem. 284: 32995-33005 [Abstract] [Full Text]  


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