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Journal of Bacteriology, April 2009, p. 2388-2391, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01594-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Differential Binding Profiles of StpA in Wild-Type and hns Mutant Cells: a Comparative Analysis of Cooperative Partners by Chromatin Immunoprecipitation-Microarray Analysis{triangledown} ,{dagger}

Ebru Uyar,1 Ken Kurokawa,2 Mika Yoshimura,1 Shu Ishikawa,1 Naotake Ogasawara,1* and Taku Oshima1

Graduate School of Information Science, Nara Institute of Science and Technology, Nara 630-0101, Japan,1 Graduate School of Biological Information, Tokyo Institute of Technology, Yokohama 226-8503, Japan2

Received 11 November 2008/ Accepted 7 January 2009

We performed chromatin immunoprecipitation-microarray analysis to investigate differences in function between StpA and H-NS in Escherichia coli cells. StpA binding regions essentially overlap those of H-NS in wild-type cells, while they are reduced to one-third in the hns mutant. The H-NS binding profile was unaffected by stpA inactivation.

* Corresponding author. Mailing address: Department of Bioinformatics and Genomics, Graduate School of Information Science, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara 630-0101, Japan. Phone: 81-743-72-5430. Fax: 31-743-72-5439. E-mail: nogasawa{at}bs.naist.jp

{triangledown} Published ahead of print on 16 January 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, April 2009, p. 2388-2391, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01594-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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