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Journal of Bacteriology, April 2009, p. 2649-2655, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01663-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

YbeY, a Heat Shock Protein Involved in Translation in Escherichia coli{triangledown}

Aviram Rasouly, Miriam Schonbrun, Yotam Shenhar, and Eliora Z. Ron*

Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel

Received 25 November 2008/ Accepted 24 January 2009

Here we provide evidence that YbeY, a conserved heat shock protein with unknown function, is involved in the translation process. ybeY deletion mutants are temperature sensitive and have a significantly reduced thermotolerance. Nonetheless, there appears to be no damage of the protein quality control of mature polypeptides, as the levels of chaperones and proteases are normal and there is no accumulation of aggregates. Rather, the mutation results in a significant reduction in the level of polysomes, and upon a shift to a restrictive temperature (42°C), there is an immediate and severe slowdown of translation. Taken together, the data indicate that YbeY is an important factor for bacterial translation even at 37°C but becomes essential at high temperatures.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel. Phone: 972-3-6409379. Fax: 972-3-6414139. E-mail: eliora{at}post.tau.ac.il

{triangledown} Published ahead of print on 30 January 2009.

Journal of Bacteriology, April 2009, p. 2649-2655, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01663-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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