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Journal of Bacteriology, April 2009, p. 2834-2842, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.00817-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Role of Acinetobacter baylyi Crc in Catabolite Repression of Enzymes for Aromatic Compound Catabolism{triangledown}

Tina Zimmermann,1 Tobias Sorg,2 Simone Yasmin Siehler,3 and Ulrike Gerischer4*

Vifor Pharma AG, Rechenstrasse 37, CH-9000 St. Gallen, Switzerland,1 Institute of Molecular Biology and Tumor Research, Philipps-University Marburg, Emil Mannkopff-Str. 2, 35032 Marburg, Germany,2 Universitätsfrauenklinik und Poliklinik, University of Ulm, Ulm, Germany,3 Institute of Microbiology and Biotechnology, University of Ulm, D-89069 Ulm, Germany4

Received 10 June 2008/ Accepted 27 January 2009

Here, we describe for the first time the Crc (catabolite repression control) protein from the soil bacterium Acinetobacter baylyi. Expression of A. baylyi crc varied according to the growth conditions. A strain with a disrupted crc gene showed the same growth as the wild type on a number of carbon sources. Carbon catabolite repression by acetate and succinate of protocatechuate 3,4-dioxygenase, the key enzyme of protocatechuate breakdown, was strongly reduced in the crc strain, whereas in the wild-type strain it underwent strong catabolite repression. This strong effect was not based on transcriptional regulation because the transcription pattern of the pca-qui operon (encoding protocatechuate 3,4-dioxygenase) did not reflect the derepression in the absence of Crc. pca-qui transcript abundance was slightly increased in the crc strain. Lack of Crc dramatically increased the mRNA stability of the pca-qui transcript (up to 14-fold), whereas two other transcripts (pobA and catA) remained unaffected. p-Hydroxybenzoate hydroxylase activity, encoded by pobA, was not significantly different in the absence of Crc, as protocatechuate 3,4-dioxygenase was. It is proposed that A. baylyi Crc is involved in the determination of the transcript stability of the pca-qui operon and thereby effects catabolite repression.

* Corresponding author. Mailing address: Institute of Microbiology and Biotechnology, University of Ulm, D-89069 Ulm, Germany. Phone: 49-731-5022715. Fax: 49-731-5022719. E-mail: ulrike.gerischer{at}uni-ulm.de

{triangledown} Published ahead of print on 6 February 2009.

Journal of Bacteriology, April 2009, p. 2834-2842, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.00817-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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