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Journal of Bacteriology, April 2009, p. 2871-2875, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01747-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Modulation of Deoxysugar Transfer by the Elloramycin Glycosyltransferase ElmGT through Site-Directed Mutagenesis{triangledown}

Angelina Ramos,{dagger} Carlos Olano, Alfredo F. Braña, Carmen Méndez, and José A. Salas*

Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain

Received 15 December 2008/ Accepted 11 February 2009

The glycosyltransferase ElmGT from Streptomyces olivaceus is involved in the biosynthesis of the antitumor drug elloramycin, and it has been shown to possess a broad deoxysugar recognition pattern, being able to transfer different L- and D-deoxysugars to 8-demethyl-tetracenomycin C, the elloramycin aglycone. Site-directed mutagenesis in residues L309 and N312, located in the {alpha}/β/{alpha} motif within the nucleoside diphosphate-sugar binding region, can be used to modulate the substrate flexibility of ElmGT, making it more precise for transfer of specific deoxysugars.

* Corresponding author. Mailing address: Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain. Phone and fax: 34 985 103652. E-mail: jasalas{at}uniovi.es

{triangledown} Published ahead of print on 20 February 2009.

{dagger} Present address: Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, 24006 León, Spain.

Journal of Bacteriology, April 2009, p. 2871-2875, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01747-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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