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Journal of Bacteriology, April 2009, p. 2884-2887, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01337-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Rv1712 Locus from Mycobacterium tuberculosis H37Rv Codes for a Functional CMP Kinase That Preferentially Phosphorylates dCMP{triangledown} ,{dagger}

Caroline Thum,1,2 Cristopher Z. Schneider,1 Mario S. Palma,3 Diógenes S. Santos,1* and Luiz A. Basso1*

Centro de Pesquisas em Biologia Molecular e Funcional, Instituto Nacional de Ciência e Tecnologia em Tuberculose, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Av. Ipiranga 6681, Porto Alegre, RS 90619-900, Brazil,1 Programa de Pós-Graduação em Biologia Celular e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul, Av. Ipiranga 6681, Porto Alegre, RS 90619-900, Brazil,2 Laboratório de Biologia Estrutural e Zooquímica, Centro de Estudos de Insetos Sociais, Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista—Rio Claro, SP 13506-900, Brazil3

Received 24 September 2008/ Accepted 26 January 2009

The Mycobacterium tuberculosis cmk gene, predicted to encode a CMP kinase (CMK), was cloned and expressed, and its product was purified to homogeneity. Steady-state kinetics confirmed that M. tuberculosis CMK is a monomer that preferentially phosphorylates CMP and dCMP by a sequential mechanism. A plausible role for CMK is discussed.

* Corresponding author. Mailing address for Luiz A. Basso: Av. Ipiranga 6681, Tecnopuc-Prédio 92A, Porto Alegre, RS 90619-900, Brazil. Phone and fax: 55 51 33203629. E-mail: luiz.basso{at}pucrs.br. Mailing address for Diógenes S. Santos: Av. Ipiranga 6681, Tecnopuc-Prédio 92A, Porto Alegre, RS 90619-900, Brazil. Phone and fax: 55 51 33203629. E-mail: diogenes{at}pucrs.br

{triangledown} Published ahead of print on 30 January 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, April 2009, p. 2884-2887, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01337-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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