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Journal of Bacteriology, May 2009, p. 2909-2916, Vol. 191, No. 9
0021-9193/09/$08.00+0     doi:10.1128/JB.01708-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Chemotaxis to Pyrimidines and Identification of a Cytosine Chemoreceptor in Pseudomonas putida{triangledown} ,{dagger}

Xianxian Liu, Piper L. Wood, Juanito V. Parales, and Rebecca E. Parales*

Department of Microbiology, University of California, Davis, California 95616

Received 6 December 2008/ Accepted 13 February 2009

We developed a high-throughput quantitative capillary assay and demonstrated that Pseudomonas putida strains F1 and PRS2000 were attracted to cytosine, but not thymine or uracil. In contrast, Pseudomonas aeruginosa PAO1 was not chemotactic to any pyrimidines. Chemotaxis assays with a mutant strain of F1 in which the putative methyl-accepting chemotaxis protein-encoding gene Pput_0623 was deleted revealed that this gene (designated mcpC) encodes a chemoreceptor for positive chemotaxis to cytosine. P. putida F1 also responded weakly to cytidine, uridine, and thymidine, but these responses were not mediated by mcpC. Complementation of the F1 {Delta}mcpC mutant XLF004 with the wild-type gene restored chemotaxis to cytosine. In addition, introduction of this gene into P. aeruginosa PAO1 conferred the ability to respond to cytosine. To our knowledge, this is the first report of a chemoreceptor for cytosine.

* Corresponding author. Mailing address: Department of Microbiology, 226 Briggs Hall, 1 Shields Ave., University of California, Davis, CA 95616. Phone: (530) 754-5233. Fax: (530) 752-9014. E-mail: reparales{at}ucdavis.edu

{triangledown} Published ahead of print on 27 February 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, May 2009, p. 2909-2916, Vol. 191, No. 9
0021-9193/09/$08.00+0     doi:10.1128/JB.01708-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liu, X., Parales, R. E. (2009). Bacterial Chemotaxis to Atrazine and Related s-Triazines. Appl. Environ. Microbiol. 75: 5481-5488 [Abstract] [Full Text]  


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