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Journal of Bacteriology, May 2009, p. 3172-3176, Vol. 191, No. 9
0021-9193/09/$08.00+0     doi:10.1128/JB.00040-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Activation and Repression at the Escherichia coli ynfEFGHI Operon Promoter{triangledown}

Meng Xu, Stephen J. W. Busby,* and Douglas F. Browning

School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom

Received 14 January 2009/ Accepted 15 February 2009

Induction of the Escherichia coli K-12 ynfEFGHI operon in response to anaerobiosis is repressed by nitrate ions. In this study, we show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter –10 element. Electromobility shift assays show that NarL does not prevent RNA polymerase binding and suggest that repression may involve a quaternary NarL-FNR-RNA polymerase-promoter complex.

* Corresponding author. Mailing address: School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom. Phone: 44-121-414-5439. Fax: 44-121-414-5925. E-mail: s.j.w.busby{at}bham.ac.uk

{triangledown} Published ahead of print on 27 February 2009.

Journal of Bacteriology, May 2009, p. 3172-3176, Vol. 191, No. 9
0021-9193/09/$08.00+0     doi:10.1128/JB.00040-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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