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J Bacteriol. 1961 July; 82(1): 43-47
Copyright ©, 1961, The Williams & Wilkins Company. All Rights Reserved.

PRODUCTION OF VIBRIO EL TOR TOXIN IN A LIQUID MEDIUM

^a Department of Bacteriology, 406th Medical General Laboratory, U. S. Army Medical Center, Japan

ABSTRACT

WATANABE, YOSHIKAZU (U. S. Army Medical Center, Japan), AND OSCAR FELSENFELD. Production of vibrio El Tor toxin in a liquid medium. J. Bacteriol. 82:43–47. 1961.—High toxin titers (as measured by mouth lethality and hemolytic activity) were obtained from culture supernatants of strains of El Tor vibrio isolated from patients in Indonesia. Cultivation of the organism in vitro revealed that the concentration of ferrous ion in the medium was critical for the production of maximal yields of toxin.

Toxins produced under appropriate conditions were antigenic in rabbits and produced antibodies capable of neutralizing both the hemolytic and lethal actions in multiple proportion. Suggestive evidence has been obtained that the hemolytic and lethal toxins may be separate molecules.

¹ Present address: Department of Microbiology, The University of Texas Medical Branch, Galveston.

² Present address: Cholera Research Laboratory, South-East Asia Treaty Organization, Bangkok, Thailand.