This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sacks, L. E. Articles by Alderton, G. Search for Related Content PubMed PubMed Citation Articles by Sacks, L. E. Articles by Alderton, G.

 Previous Article  |  Next Article 

J Bacteriol. 1961 September; 82(3): 331-341
Copyright ©, 1961, The Williams & Wilkins Company. All Rights Reserved.

BEHAVIOR OF BACTERIAL SPORES IN AQUEOUS POLYMER TWO-PHASE SYSTEMS

Western Regional Research Laboratory, U. S. Department of Agriculture, Albany, California

ABSTRACT

SACKS, L. E. (Western Regional Research Laboratory, Albany, California), AND GORDON ALDERTON. Behavior of bacterial spores in aqueous polymer two-phase systems. J. Bacteriol. 82:331–341. 1961.—Free spores of various species of the Bacillaceae enter the upper phase in a two-phase aqueous polymer system containing polyethylene glycol 4000 and potassium phosphate. Vegetative cells appear in the lower phase and subcellular debris is concentrated chiefly at the interface. The high selectivity of the upper phase for spores makes possible the preparation of greatly enriched concentrates of spores directly from complex natural materials, such as soil and feces. Attraction of spores for the upper phase is not diminished by autoclaving and the release of dipicolinic acid. Loss of attraction of spores for the upper phase is associated in time with the shedding of the spore coat, upon outgrowth. The partition behavior of one preparation of Bacillus megaterium showed the presence of two classes of spores.

This article has been cited by other articles:

• Seale, R. B., Flint, S. H., McQuillan, A. J., Bremer, P. J. (2008). Recovery of Spores from Thermophilic Dairy Bacilli and Effects of Their Surface Characteristics on Attachment to Different Surfaces. Appl. Environ. Microbiol. 74: 731-737 [Abstract] [Full Text]  
• Mazzotta, A. S., Montville, T. J. (1999). Characterization of Fatty Acid Composition, Spore Germination, and Thermal Resistance in a Nisin-Resistant Mutant of Clostridium botulinum 169B and in the Wild-Type Strain. Appl. Environ. Microbiol. 65: 659-664 [Abstract] [Full Text]