This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Meyer, M. E. Articles by Cameron, H. S. Search for Related Content PubMed PubMed Citation Articles by Meyer, M. E. Articles by Cameron, H. S.

 Previous Article  |  Next Article 

J Bacteriol. 1961 September; 82(3): 387-395
Copyright ©, 1961, The Williams & Wilkins Company. All Rights Reserved.

METABOLIC CHARACTERIZATION OF THE GENUS BRUCELLA I.

Statistical Evaluation of the Oxidative Rates by Which Type I of Each Species Can Be Identified

^a School of Veterinary Medicine, University of California, Davis, California

ABSTRACT

MEYER, MARGARET E. (University of California, Davis), AND H. S. CAMERON. Metabolic characterization of the genus Brucella. I. Statistical evaluation of the oxidative rates by which type I of each species can be identified. J. Bacteriol. 82:387–395. 1961.—The oxidative uptake rates on 11 amino acid and seven carbohydrate substrates were determined for 75 strains of brucellae that had been identified by the conventional determinative methods as Brucella melitensis type I, Brucella abortus type I, or Brucella suis type I. By calculating the standard deviation of the oxidative rates, it was demonstrated that a metabolic pattern that is characteristic and definitive for each of the species was formed by their differential oxidative utilization of substrate groups, and that qualitative as well as quantitative metabolic differences exist among the Brucella species. B. melitensis oxidized L-alanine, L-asparagine, and L-glutamic acid, but not L-arginine, DL-citrulline, L-lysine, DL-ornithine, L-arabinose, D-galactose, D-ribose, or D-xylose. B. abortus differed qualitatively from B. melitensis in that it oxidized the carbohydrate substrates. B. suis differed quantitatively from both of these species in its consistently low oxidative rates of L-alanine, L-asparagine, and L-glutamic acid, and its high rates of utilization of the carbohydrate substrates. It differed qualitatively in that it oxidized the four amino acid substrates that are components of the urea cycle.

¹ These papers represent part of a thesis submitted by the senior author to the Graduate Division of the University of California, in partial fulfillment of the requirements of the Ph.D. degree.

This article has been cited by other articles:

• Foster, G., Osterman, B. S., Godfroid, J., Jacques, I., Cloeckaert, A. (2007). Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int. J. Syst. Evol. Microbiol. 57: 2688-2693 [Abstract] [Full Text]