This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Soffer, R. L. Search for Related Content PubMed PubMed Citation Articles by Soffer, R. L.

 Previous Article  |  Next Article 

J Bacteriol. 1961 October; 82(4): 471-478
Copyright ©, 1961, The Williams & Wilkins Company. All Rights Reserved.

ENZYMATIC EXPRESSION OF GENETIC UNITS OF FUNCTION CONCERNED WITH GALACTOSE METABOLISM IN ESCHERICHIA COLI

^a Department of Genetics, Stanford University, Palo Alto, California

ABSTRACT

SOFFER, RICHARD L. (Stanford University, Palo Alto, Calif.). Enzymatic expression of genetic units of function concerned with galactose metabolism in Escherichia coli. J. Bacteriol. 82:471–478. 1961.—A series of genetically characterized galactose-negative mutants of Escherichia coli strain K12 were studied with respect to the enzymes of the Leloir pathway for utilization of this sugar. The functions pertaining to the three genetically defined cistrons were found to relate, respectively, to the expression of galactokinase, galactose 1-phosphate uridyl transferase, and uridine diphosphate galactose 4-epimerase. Mutants defective in the latter enzyme represent a new biochemical class in this strain of E. coli. Most of the mutants defective in galactokinase were found to exhibit significantly elevated levels of galactose 1-phosphate uridyl transferase as compared with the wild type when grown under conditions of induction. A suppressed transferase-deficient mutant was found to have regained this enzymatic activity.

¹ Present address: Department of Medicine, New York University Medical School, 550 First Avenue, New York 16, N. Y. The work reported here was done during the tenure of a Post-doctoral Fellowship under the auspices of the American Cancer Society.

This article has been cited by other articles:

• Chugani, S. A., Parsek, M. R., Chakrabarty, A. M. (1998). Transcriptional Repression Mediated by LysR-Type Regulator CatR Bound at Multiple Binding Sites. J. Bacteriol. 180: 2367-2372 [Abstract] [Full Text]