This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hinze, H. C. Articles by Walker, D. L. Search for Related Content PubMed PubMed Citation Articles by Hinze, H. C. Articles by Walker, D. L.

 Previous Article  |  Next Article 

J Bacteriol. 1961 October; 82(4): 498-504
Copyright ©, 1961, The Williams & Wilkins Company. All Rights Reserved.

VARIATION OF HERPES SIMPLEX VIRUS IN PERSISTENTLY INFECTED TISSUE CULTURES

Department of Medical Microbiology, University of Wisconsin Medical School, Madison, Wisconsin

ABSTRACT

HINZE, HARRY C. (University of Wisconsin, Madison), AND DUARD L. WALKER. Variation of herpes simplex virus in persistently infected tissue cultures. J. Bacteriol. 82:498–504. 1961.—Cultures of human conjunctiva, HeLa, and KB cells infected with herpes simplex virus (HF strain) were cultured for prolonged periods in medium containing low levels of antibody. After continuous culture of the infected cell lines for 6 months, two major changes were noted in the character of the virus present in the cultures. These changes consisted of an alteration in the type of cytopathic effect produced by the virus and marked loss of virulence for mice. Further study of the virus from the cultures revealed that it was still antigenically similar to the original strain of herpes virus. It was found that the variant produced an altered, proliferative type of cytopathic effect only in the presence of herpes antibody. The variant multiplied more slowly in human conjunctiva cells in culture than did the parent virus, and the variant had lost practically all capacity for multiplication in the brain of mice.