This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Griffin, C. W. Articles by Warner, G. S. Search for Related Content PubMed PubMed Citation Articles by Griffin, C. W. Articles by Warner, G. S.

 Previous Article  |  Next Article 

J Bacteriol. 1961 October; 82(4): 534-537
Copyright ©, 1961, The Williams & Wilkins Company. All Rights Reserved.

LABELING PROCEDURES EMPLOYING CRYSTALLINE FLUORESCEIN ISOTHIOCYANATE¹

Research and Development Laboratory, Baltimore Biological Laboratory, and Biological Research Laboratories, Hynson, Westcott
Dunning, Inc., Baltimore, Maryland

ABSTRACT

GRIFFIN, CHARLES W. (Baltimore Biological Laboratory, Baltimore, Md.), THEODORE R. CARSKI, AND GEORGE S. WARNER. Labeling procedures employing crystalline fluorescein isothiocyanate. J. Bacteriol. 82:534–537. 1961.—The effect of altering the ratio of dye to protein in the labeling of three immune globulins with crystalline fluorescein isothiocyanate was the purpose of the study. Direct staining of group A streptococci and rabies-infected mouse brain, and indirect staining of Treponema pallidum (Nichols strain) comprised the three immunological systems selected for investigation. Maximal specific staining and minimal nonspecific background staining were demonstrated by the use of low dye-to-protein labeling ratios.

¹ This research was performed under contract SA-43-ph-2442 for the National Institutes of Health, U. S. Public Health Service.