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J Bacteriol. 1961 December; 82(6): 808-814
Copyright © 1961, The Williams & Wilkins Company. All Rights Reserved.

SPHEROPLASTS OF THE YEAST CANDIDA UTILIS

G. Svihla, F. Schlenk and J. L. Dainko

Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois

ABSTRACT

SVIHLA, G. (Argonne National Laboratory, Argonne, Ill.), F. SCHLENK, AND J. L. DAINKO. Spheroplasts of the yeast Candida utilis. J. Bacteriol. 82:808–814. 1961.—The formation and properties of spheroplasts of the yeast Candida utilis, produced by digestion of the cell walls with snail gut juice (Helix pomatia) in isotonic medium, were studied by phase, interference, and ultraviolet microscopy, and tracer techniques. Cells cultivated in the presence of L-methionine, S-methyl-L-methionine, and L-homocysteine (5 µmoles/ml) were more susceptible to enzyme action than ordinary cells. These cells contain increased amounts of S-adenosylmethionine in the vacuole which facilitates ultraviolet microscopy. Spheroplasts are stable in 0.6 M KCl containing 0.04 M phosphate, but are disintegrated by water, sonic vibration, or ultraviolet irradiation. Exposure in isotonic medium to 6 x 104 erg/mm2 energy at 253 mµ breaks the cytoplasmic membrane and liberates the vacuole with membrane intact. The vacuolar membrane may be broken with about three times the initial dose of ultraviolet.

J Bacteriol. 1961 December; 82(6): 808-814
Copyright © 1961, The Williams & Wilkins Company. All Rights Reserved.





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