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J Bacteriol. 1962 January; 83(1): 14-19
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

A NEW METHOD FOR THE TURBIDIMETRIC MEASUREMENT OF BACTERIAL DENSITY

Masami Kurokawa, Motoichi Hatano, Noboru Kashiwagi, Tateo Saito, Setsuji Ishida and Reiko Homma

National Institute of Health, Tokyo
Department of Bacteriology, Kanazawa University School of Medicine, Kanazawa
2nd Surgical Department, Chiba University School of Medicine, Chiba, Japan

ABSTRACT

KUROKAWA, MASAMI (National Institute of Health, Tokyo, Japan), MOTOICHI HATANO, NOBORU KASHIWAGI, TATEO SAITO, SETSUJI ISHIDA, AND REIKO HOMMA. A new method for the turbidimetric measurement of bacterial density. J. Bacteriol. 83:14–19. 1962.—It was demonstrated that by a combination of two types of metameter of transmittance (T), –log T (0.0 ≤ T ≤ 0.5) and 0.6 + log (1 – T) (0.5 ≤ T ≤ 1.0), the optical density thus expressed showed a linear relationship to bacterial density or cell concentration over the whole range of T, except at the extreme ends. This allows measurement of relative bacterial density and cell concentration, and eliminates unnecessary sampling and dilution. In this respect, the proposed method seems superior to the method based upon Beer's law. The possible error derived from the fact that organisms may vary in size and shape at different stages of their growth is a fault common to all known methods of turbidimetric measurement, and is not peculiar to the method presented here.

J Bacteriol. 1962 January; 83(1): 14-19
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.





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