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J Bacteriol. 1962 February; 83(2): 276-283
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

ULTRAVIOLET MICROSCOPY OF BUDDING SACCHAROMYCES

^a Biological Research Laboratory, Southern Illinois University, Carbondale, Illinois

ABSTRACT

MCCLARY, DAN O. (Southern Illinois University, Carbondale), WILBERT D. BOWERS, JR., AND GLENDON R. MILLER. Ultraviolet microscopy of budding Saccharomyces. J. Bacteriol. 83:276–283. 1962.—Synchronous cell division was obtained in Saccharomyces by transferring starved cells into nutrient medium. Ultraviolet microscopy and Giemsa-stained preparations of these cells showed nuclear division to occur in the mother cell early in the budding process. The divided nucleus passed into the neck between the mother cell and the bud, and either fused together again or the two parts became so closely associated that one continuous dumbbell-shaped body was seen which seemed to divide by constriction. This effect was probably due to the retention of the intact nuclear membrane until nuclear division was otherwise complete.

The nuclear apparatus lies outside the vacuole. The extent to which these bodies function together cannot be determined by the techniques employed in this work.

Parallel experiments on meristematic cells of onion root tips show the reliability of these cytological methods.

¹ Research participant sponsored by the National Science Foundation Undergraduate Research Participation Program.