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J Bacteriol. 1962 February; 83(2): 418-422
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

NUTRITIONAL REQUIREMENTS FOR HYDROGENOMONAS EUTROPHA

National Institute of Allergy and Infectious Diseases, U. S. Public Health Service, Bethesda, Maryland

ABSTRACT

REPASKE, ROY (National Institute of Allergy and Infectious Diseases, Bethesda, Md.). Nutritional requirements for Hydrogenomonas eutropha. J. Bacteriol. 83: 418–422. 1962.—A simple apparatus for the autotrophic cultivation of Hydrogenomonas eutropha in 100-ml shake cultures is described. Nitrogen, in the form of ammonium, nitrate, or urea, was used for growth; nitrite could not be utilized. Optimal growth occurred at pH 6.4 to 6.8 at 30 C. H. eutropha grew best in an atmosphere containing 15 to 25% oxygen and 10% carbon dioxide. Below these concentrations each of the gases was limiting. Growth was shown to be dependent on iron, and the rate of growth was a function of iron concentration and its state of oxidation.