This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Perry, D. Articles by Slade, H. D. Search for Related Content PubMed PubMed Citation Articles by Perry, D. Articles by Slade, H. D.

 Previous Article  |  Next Article 

J Bacteriol. 1962 March; 83(3): 443-449
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

TRANSFORMATION OF STREPTOCOCCI TO STREPTOMYCIN RESISTANCE

^a Department of Microbiology, Northwestern University Medical School, Chicago, Illinois

ABSTRACT

PERRY, DENNIS (Northwestern University Medical School, Chicago, Ill.) AND HUTTON D. SLADE. Transformation of streptococci to streptomycin resistance. J. Bacteriol. 83:443–449. 1962.—A total of 43 strains of streptococci, belonging to serological groups A, B, C, D, E, F, G, H, K, L, M, N, O, and Q, and three ungroupable strains were exposed to deoxyribonucleic acid (DNA) extracted from streptomycin-resistant streptococci belonging to groups A, C, D, G, H, and ungroupable strains. Transformation to streptomycin resistance was obtained with streptococci belonging to groups F, H, and O and a serologically unclassifiable streptococcus. The transformable streptococci incorporated DNA from both homologous and heterologous groups and strains of streptococci. Certain strains within a group served as DNA donors but not as DNA recipients, and certain strains served as DNA donors for some strains but not for others. Even within an individual group, not all strains were transformable. The number of transformants was influenced by the source of DNA and the DNA recipient strain used. Substances which affect cell permeability did not enable transformation to occur. Radioisotope studies with P³²-labelled DNA showed that nontransformable strains incorporated amounts of DNA comparable to the transformable strains.

¹ Established Investigator of the American Heart Association.

This article has been cited by other articles:

• Leonard, C. G., Corley, D., Cole, R. M. (1966). Transformation of Auxotrophic Mutants of Group H Streptococci. Science 152: 1255-1256 [Abstract]