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J Bacteriol. 1962 March; 83(3): 470-474
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

A DEFINED AGAR MEDIUM FOR GENETIC TRANSFORMATION OF NEISSERIA MENINGITIDIS

^a Department of Microbiology and Immunology, School of Medicine, Marquette University, Milwaukee, Wisconsin

ABSTRACT

CATLIN, B. WESLEY (Marquette University, Milwaukee, Wis.) AND GERTRUDE M. SCHLOER. A defined agar medium for genetic transformation of Neisseria meningitidis. J. Bacteriol. 83:470–474. 1962.—An agar medium was developed for use in quantitative genetic studies of Neisseria meningitidis strain 15. It contains eight inorganic salts, sodium citrate, sodium lactate, arginine, cysteine, glycine, sodium glutamate, and purified agar. Abundant surface growth in the absence of supplemental carbon dioxide was obtained during 50 serial subcultures. A close correspondence was found between numbers of parental type colonies developing on the defined medium and on a complex medium. Cells subcultured serially three or four times on defined agar medium and placed directly into a solution of transforming deoxyribonucleic acid in defined liquid medium were susceptible to transformation without additional supplements. Of the treated population, 0.1 to 0.3% of the cells were transformed to streptomycin resistance.

¹ Present address: Department of Veterinary Science, School of Agriculture, University of Wisconsin, Madison.

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