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J Bacteriol. 1962 April; 83(4): 720-726
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

NUTRITIONAL FACTORS STIMULATING THE FORMATION OF LYSINE DECARBOXYLASE IN ESCHERICHIA COLI

Andrew Maretzki1 and M. F. Mallette

a Department of Agricultural and Biological Chemistry, Pennsylvania State University, University Park, Pennsylvania

ABSTRACT

MARETZKI, ANDREW (Pennsylvania State University, University Park) AND M. F. MALLETTE. Nutritional factors stimulating the formation of lysine decarboxylase in Escherichia coli. J. Bacteriol. 83:720–726. 1962 — Inclusion of complex nitrogen sources in the induction medium was shown to be necessary for the synthesis of appreciable amounts of L-lysine decarboxylase by Escherichia coli B. Hy-case, a commercial acid hydrolyzate of casein, was especially effective in enzyme production, which was assayed manometrically after lysis of the bacteria from without by bacteriophage. Partial fractionation of the Hy-case, identification of the free amino acids, and addition of these amino acids to test media revealed stimulatory effects by methionine, threonine, proline, leucine, and tyrosine. A full complement of amino acids did not match the enzyme levels reached in the presence of Hy-case. Certain peptide fractions obtained from this mixture supplemented the effects of the amino acids in such a way as to suggest direct incorporation of peptide rather than transport or protective roles. Added purines, pyrimidines, iron, and water-soluble vitamins were without effect. Neither carbohydrates nor phosphorylated materials could be detected in the stimulatory fractions.

FOOTNOTES

1 Present address: The Medical School, University of Puerto Rico, San Juan, Puerto Rico.

J Bacteriol. 1962 April; 83(4): 720-726
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.





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