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J Bacteriol. 1962 April; 83(4): 727-735
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE IN ENTEROBACTERIACEAE IV.

Interactions between Resistance Transfer Factor and F-Factor in Escherichia coli K-12

Department of Bacteriology, Keio University School of Medicine, Tokyo, Japan

ABSTRACT

WATANABE, T. (Keio University, Tokyo, Japan) AND T. FUKASAWA. Episome-mediated transfer of drug resistance in Enterobacteriaceae. IV. Interactions between resistance transfer factor and F-factor in Escherichia coli K-12. J. Bacteriol. 83:727–735. 1962. — Resistance transfer factor (RTF) present in F⁺ and F'⁺ strains was found to suppress completely the transfer of F-factor, F', and host chromosomes. On the other hand, the presence of RTF in Hfr strains was found to reduce the frequencies of recombination to about 10^–2 of those in Hfr strains, regardless of the types of Hfr. RTF present in an F⁺ strain with colicinogenic factor E₁ inhibited the transfer of the colicinogenic factor, whereas the transfer of colicinogenic factor I was not suppressed by RTF. A hypothesis that the genetic determinant of RTF controlling its "transfer system" is epistatic to that of F-factor is presented. It was also shown that F-factor present in recipients slightly suppresses the acceptance of RTF and that RTF present in recipients slightly reduces the acceptance of F-factor, F', and host chromosomes. On the other hand, the acceptance of RTF by recipients in which RTF is present was found to be about 1% of that in recipients without RTF.